Page 43 - Demo

Son ara%u015ft%u0131rmalar, bitki kaynakl%u0131 ekzosomlar%u0131n cilt ile ilgili etkilerini, yani antioksidan ve anti-inflamatuvar %u00f6zellikleri %u00fczerine yap%u0131lan %u00e7al%u0131%u015fmalar da dahil olmak %u00fczere faydalar%u0131n%u0131 g%u00f6stermi%u015ftir. Bitki kaynakl%u0131 ekzosomlar, bitki h%u00fccreleri taraf%u0131ndan salg%u0131lanan sinyal maddeleri i%u00e7ermektedir. Bitkilerden safla%u015ft%u0131r%u0131lan ekzosomlar%u0131n memeli kaynakl%u0131 ekzosomlara k%u0131yasla hi%u00e7bir toksisiteye sahip olmad%u0131%u011f%u0131 bilinmektedir. %u00c7ok say%u0131daki avantaj ve etkinlikleri nedeniyle ila%u00e7larda, kozmetiklerde ve g%u0131dalarda kullan%u0131labilmektelerdir. Eksozomlar%u0131 izole etmek i%u00e7in kullan%u0131lan ultra santrif%u00fcj y%u00f6nteminde olduk%u00e7a pahal%u0131 ekipman ve uzun ayr%u0131%u015ft%u0131rma s%u00fcresine ihtiya%u00e7 duyulmaktad%u0131r. Te%u011fetsel ak%u0131%u015f filtrasyonu ekipman kurulumu a%u00e7%u0131s%u0131ndan karma%u015f%u0131kt%u0131r. CICA EXOSOME bitki kaynakl%u0131 eksozomlar%u0131n stabilitesini sa%u011flamaktad%u0131r.Centella asiatica uzun zamand%u0131r t%u0131bbi bir bitki olarak kullan%u0131lmaktad%u0131r ve aktif bile%u015fenleri aras%u0131nda pentasiklik triterpenler bulunmaktad%u0131r: asiatikosit, madekasosit, asiatik asit ve madekasik asit. Bu bile%u015fenler cilt h%u00fccrelerinde kolajen %u00fcretimini te%u015fvik etmedeki etkinlikleri, antioksidan aktiviteleri, ya%u015flanma kar%u015f%u0131t%u0131 %u00f6zellikleri, anti-inflamatuvar etkileri, fotoprotektif etkileri ve yara iyile%u015ftirme yetenekleri ile bilinmektedir ve yara iyile%u015ftirmede kullan%u0131lmaktad%u0131r. Bu %u00e7al%u0131%u015fmada, Centella asiatica%u2019dan (CICA) ekzosomlar ekstrakte edilmi%u015ftir ve kozmetik hammaddeleri olarak potansiyelleri de%u011ferlendirilmi%u015ftir.C. asiatica Ekzosomunun anti-inflamatuvar etkisinin do%u011frulanmas%u0131Transkripsyon fakt%u00f6r%u00fc n%u00fckleer fakt%u00f6r-%u03baB (NF-%u03baB), proinflamatuvar gen ind%u00fcksiyonunun merkezi arac%u0131s%u0131d%u0131r. Bu deneyde, insan epidermal keratinosit (HEKa) h%u00fccreleri, NF-%u03baB ekspresyonunu aktive etmek i%u00e7in UVB%u2019ye (20 mJ/cm%u00b2) maruz b%u0131rak%u0131lm%u0131%u015ft%u0131r. H%u00fccreler, NF-%u03baB ekspresyon seviyelerini kar%u015f%u0131la%u015ft%u0131rmak i%u00e7in de%u011fi%u015fen konsantrasyonlarda Cica ekstrakt%u0131 veya Cica ekzosomu veya %0,01 Allantoin ile muamele edilmi%u015ftir. Bu sonu%u00e7, kontrol ile kar%u015f%u0131la%u015ft%u0131r%u0131ld%u0131%u011f%u0131nda, Cica ekzosomu ile muamele edilen h%u00fccrelerin bu iki gen ekspresyonunu azaltt%u0131%u011f%u0131n%u0131 g%u00f6stermi%u015ftir, Cica ekstrakt%u0131 ile kar%u015f%u0131la%u015ft%u0131r%u0131ld%u0131%u011f%u0131nda da anti-inflamatuvar etkide daha y%u00fcksek etkili oldu%u011funu g%u00f6stermi%u015ftir. 1x107 par%u00e7ac%u0131k/ml Cica ekzosomu ile muamele, NF-%u03baB sinyal yollar%u0131n%u0131n a%u015f%u0131r%u0131 aktivasyonunu inhibe ederek inflamatuvar yan%u0131tlar%u0131 bask%u0131lad%u0131%u011f%u0131 kan%u0131tlanm%u0131%u015f olan Allantoinden daha y%u00fcksek etkinlik g%u00f6stermi%u015ftir (%u015eekil 1).Recent research has shown benefits of plantderived exosomes, including studies on their skin-related effects, i.e. antioxidant and anti-inflammatory properties. Plant-derived exosomes contain signaling substances secreted by plant cells. Exosomes purified from plants are known to have no toxicity compared to mammalian-derived exosomes. Due to their numerous advantages and activities, they can be utilized in pharmaceuticals, cosmetics, and food.The ultracentrifugation method of isolating exosomes requires highly expensive equipment and long separation times. Tangential flow filtration is complicated in terms of equipment setup. CICA EXOSOME secures stability of plant-derived exosomes.Centella asiatica has long been used as a medicinal plant, active components of which include pentacyclic triterpene: asiaticoside, madecassoside, asiatic acid, and madecassic acid. These components are known for their efficacy in promoting collagen production in skin cells, antioxidant activity, antiaging properties, anti-inflammatory effects, photoprotective effects, and wound healing capabilities, and are used for wound healing. In this study, exosomes were extracted from Centella asiatica (CICA) and evaluated for their potential as cosmetic raw materials. Confirmation of anti-inflammatory effect of C. asiatica ExosomeThe transcription factor nuclear factor-%u03baB (NF-%u03baB) is the central mediator of pro-inflammatory gene induction. In this experiment, human epidermal keratinocyte (HEKa) cells were exposed to UVB (20 mJ/cm%u00b2) to activate NF-%u03baB expression. Cells were treated with varying concentrations of Cica extract or Cica exosome, or 0.01% Allantoin to compare NF-%u03baB expression levels. The result showed that the cells treated with Cica exosome had downregulated these two gene expressions compared to the control, indicating its higher efficacy in antiinflammatory effect compared to Cica extract. Treatment with 1x107 particles/ml of Cica exosome also showed higher efficacy than Allantoin, which is proven to suppress inflammatory responses by inhibiting the overactivation of the NF-%u03baB signaling pathways (Figure 1).Makale / Article41