Page 44 - Demo

Makale / Article42C. asiatica ekzosomunun k%u0131r%u0131%u015f%u0131kl%u0131k kar%u015f%u0131t%u0131 etkisinin do%u011frulanmas%u0131: Kolajen tip I alfa 1COL1A1, kemik ve cildin ekstrasel%u00fcler matrisinin ana bile%u015feni olan tip I kollajenin ana bile%u015fenidir. Bu deneyde, insan dermal fibroblast (HDFa) h%u00fccreleri, COL1A1 ekspresyon seviyelerini kar%u015f%u0131la%u015ft%u0131rmak i%u00e7in %u00e7e%u015fitli konsantrasyonlarda Cica ekstrakt%u0131 veya Cica ekzosomu veya 20nM TGF%u03b21 ile muamele edilmi%u015ftir. Cica ekzosomu ile tedavi edilen h%u00fccreler, COL1A1 mRNA ekspresyonunu artt%u0131rm%u0131%u015ft%u0131r, ekstrakta k%u0131yasla daha y%u00fcksek k%u0131r%u0131%u015f%u0131kl%u0131k iyile%u015ftirme etkinli%u011fini do%u011frulam%u0131%u015ft%u0131r. 1x107 par%u00e7ac%u0131k/ml Cica ekzosomu ile muamele, COL1A1%u2019in mRNA ekspresyonunu artt%u0131rd%u0131%u011f%u0131 kan%u0131tlanm%u0131%u015f olan TGF%u03b21%u2019den daha y%u00fcksek etkinlik g%u00f6stermi%u015ftir (%u015eekil 2).C. asiatica ekzosomunun k%u0131r%u0131%u015f%u0131kl%u0131k kar%u015f%u0131t%u0131 etkisinin do%u011frulanmas%u0131: Matriks metalloproteinaz 1MMP veya matris metalloproteinaz, kolajeni par%u00e7alamada ve elastin i%u00e7eri%u011fini azaltmada rol oynamaktad%u0131r, ciltte k%u0131r%u0131%u015f%u0131kl%u0131klara ve esnekli%u011fin bozulmas%u0131na neden olmaktad%u0131r. Bu deneyde, insan dermal fibroblast (HDFa) h%u00fccreleri, MMP ekspresyon yolunu aktive eden ROS %u00fcretmek i%u00e7in UVB (20mJ/cm2) ile muamele edilmi%u015ftir. H%u00fccreler, MMP1 ekspresyon seviyelerini kar%u015f%u0131la%u015ft%u0131rmak i%u00e7in de%u011fi%u015fen konsantrasyonlarda Cica ekstrakt%u0131, ekzosom veya 20 nM TGF%u03b21 ile muamele edilmi%u015ftir. Cica ekzosoConfirmation of anti-wrinkle effect of C. asiatica exosome: Collagen type I alpha 1COL1A1 is a major component of type I collagen, which is the main component of the extracellular matrix of bone and skin. In this experiment, human dermal fibroblast (HDFa) cells were treated with varying concentrations of Cica extract or Cica exosome, or 20nM TGF%u03b21 to compare COL1A1 expression levels. The cells treated with Cica exosome had upregulated COL1A1 mRNA expression confirming its higher efficacy in wrinkle improvement compared to the extract. The treatment with 1x107 particles/ml of Cica exosome showed higher efficacy than TGF%u03b21, which is proven to upregulate the mRNA expression of COL1A1 (Figure 2).Confirmation of anti-wrinkle effect of C. asiatica exosome: Matrix metalloproteinase 1MMP or matrix metalloproteinase plays a role in degrading collagen and reducing elastin content, causing wrinkles and elasticity degradation in the skin. In this experiment, the human dermal fibroblast (HDFa) cells were treated with UVB (20mJ/cm2) to generate ROS, activating MMPs expression pathway. Cells were treated with varying concentrations of Cica extract, exosome, or 20 nM TGF%u03b21 to compare MMP1 expression levels. The cells treated with Cica exosome had downregulated MMP1 mRNA expression compared to